Journal: Journal of Virology

Article Title: Porcine hemagglutinating encephalomyelitis virus nucleocapsid protein targets RIG-I and IRF3 to evade IFN immunity

doi: 10.1128/jvi.02112-25

Figure Lengend Snippet: PHEV dsRNA serves as a prerequisite for RIG-I-dependent induction of type I IFN. ( A ) Schematic illustration of the proposed mechanisms by which Remdesivir (RDV) and Lopinavir (LPV) suppress PHEV replication. RdRp, RNA-dependent RNA polymerase; 3CLpro, 3C-like protease. ( B ) RDV and LPV suppress PHEV replication. N2a cells were infected with PHEV for 1 h, treated with RDV or LPV (20 μM), and harvested at 24 h post-infection for qRT-PCR analysis of PHEV mRNA levels. ( C ) QRT-PCR analysis of IFNA and IFNB1 mRNA expression as described in panel B . ( D ) QRT-PCR analysis of I RIG-I, IRF3, and IRF7 mRNA expression as described in panel B . ( E ) WB analysis of RIG-I, phosphorylated IRF3 (p-IRF3), p-IRF7, and PHEV N protein as described in panel B . ( F ) Immunostaining assay. N2a cells as indicated in panel B were harvested and immunostained with anti-dsRNA (red) and anti-RIG-I (green) antibodies, DAPI (blue). Scale bar, 10 µm. Data represent mean ± SD (** P < 0.01 and *** P < 0.001 by unpaired two-tailed Student’s t-test).

Article Snippet: The Remdesivir and Lopinavir were purchased from MCE.

Techniques: Infection, Quantitative RT-PCR, Expressing, Immunostaining, Two Tailed Test